The following protocol was used based on the staining improvements of BASSAM [150]. The desilver and impregnation steps were modified.
| Step | Time | Reagents |
| 1. Fix | 30 min | 10% acetic acid (v/v) |
| 2. Rinse | 3  2 min | water (bidest.) |
| 3. Impregnate | 20-30 min | AgNO  (1 g/l) |
+ 100  l 37% formaldehyde per 100 ml | ||
| 4. Rinse | 20 s | H  O (bidest.) |
| Thoroughly wash the gel surface and back of the gel | ||
| 5. Develop | 2-5 min | 2.5% NaCO |
| (visual control) | + 100 l 37% formaldehyde per 100 ml | |
+ Na S O  5H O (2 mg/liter) | ||
| 6. Stop | 10 min | 2% (g/v) glycine |
| + 0.5% EDTA disodium salt solution | ||
| 7. Impregnate | 10 min | 5% (v/v) glycerol |
| 8. Dry | overnight | room temperature |